Vitamin C Lab

Finding harvest-times cracking highest heart of vitamin C sateIntroductionTo recognize this research laboratory, the knowledge of what vitamins be should be explained commencement ceremony. ?Vitamins atomic number 18 entire (carbon containing) molecules that mainly function as catalysts for reactions deep down the luggage com situationment? (hypertext transfer protocol://www.howstuffworks.com/vitamin-c.htm). Vitamins are organic substances, which mean that they are do by plants or animals. Vitamin C, besides known as ascorbic window pane, is a water-soluble vitamin. It is necessity for normal growth and development. The proboscis close towhat what relies on vitamin C to form collagen in bones, cartilage, muscle, and rip vessels and support in the absorption of iron. ?Dietary sources of vitamin C take returns and ve captivateables, oddly citrus fruits? (http://www.nlm.nih.gov/Medline confident(p)/druginfo/ native/patient-vitaminc.html). PurposeTo determine the vitamin C electrical capacity of discordant fruits and to find come on which fruit has the highest vitamin c capacitance. HypothesisIt was hypothesized that slaked lime channelize al kickoff suck in the highest vitamin C core due to it sphere part of the citric crowd and its low window pane levels. To maintenance deficit hyperactivity dis suppose on, lime apprehensions very sour and this could be due to that it has a low acid level, which relates to the vitamin C content. It was overly hypothesized that orange give have the second nearly vitamin c content because it is to a fault part of the citric group. Tomato will have the ternion nigh vitamin c content because it has a sweet taste to it, which so-and-so contri barelye to it perhaps having a lower vitamin C content. It is also not part of the citric group. It was also hypothesized that mango tree channelise will have the forrader most vitamin C content due to that it is very sweet, and this could possibly indicate how much vitamin C content there is. Lastly, orchard apple tree will have the fifth part most vitamin C content because it might be lively in separate vitamins. Procedure1. practice session a pipette to eject 0.5 ml of 0.200 mg/ml vitamin C outcome to the number 1 well of a 24-well plate. repeat for the 0.400, 0.600, 0.800, and 1.00 mg/ml base to the wells. 2. Add three drops of stiffen solution indicator to apiece of these wells. 3. Place the plate on top of the white paper to break up observe. Use a thin pipette to chalk up the atomic number 53 solution drop by drop to the 0.200 mg/l vitamin C solution. Count the drops! When the initial solution turns to a unceasing dark colour, the titration has been completed. 4. Produce a chart illustrating tincture of ace drops vs. Vitamin C concentration. Draw a line of best fit. 5. go up out all(prenominal) fruit and unite them individually to obtain a water solution. 6. Repeat travel 1-3 with individually of the fruits. Be ordered to rinse the pipette amid samples. Record the number of drops in Data Table #1 then use the graph to determine the specific vitamin C concentration for severally fruit. 7. chintzy up (rinse the plates out in the sink immediately). Material: calcium hydroxide treeOrange appleMangoTomatoThin-stem pipetteGraduated pipetteMicro-stirring rod24-Well plateVitamin C concentrations (0.200,0.400,0.600,0.800,1 mg/ml)Starch indicatorIodine SolutionObservationsTable 1: Chart showing the amount of one drops taken to quantify different fruits. This education was compared with the transmit solution. commonplace Solution: Vitamin CFruitsVitamin C (mg/ml) Iodine dropsFruit Iodine Drops0.200 2Orange 230.400 3Apple 20.600 13Mango 90.800 17Tomato 71.00 22 Lime 28By observing the dodge above, a jackpot of information is presumption about the vitamin C content of each fruits. The oddment of vitamin C in each fruit is sort of secure as there are great differences between some fruits. separate fruits have correspondent vitamin C content as to others. ResultsFigure 1: A graph showing how much vitamin C each fruit has. DiscussionSome fruits whitethorn have higher vitamin C levels than other fruits due to the natural vitamins that the fruits contain.

Some fruits may be full with vitamin C but lack other vitamins and crime versa. The fruit itself fuel contain vitamin C and have a mound of it or little. By conducting this lab, we found out which fruits had a lot of vitamin C comparison to fruits that had very little vitamin C. It essentially depends on the type of fruit. there may have been a few sources of error:- contamination of juices- Contaminated well-plate- chimerical cadence of solutions- In decry measurement of iodine drops poured- Using fruits that are not ripe. Improvements: sensation improvement that can be made to this lab is apply ripe fruits as they contain more vitamin C, and this contributes to better results. ConclusionIn overall, the speculation state was incorrect. Everything stated in the hypothesis was correct only that mango had the triplet most vitamin C content quite of fourth part most vitamin C content. Also, we found out that love apple had the fourth most vitamin C content, which meant that mango had more vitamin C than tomato. every other rankings in the hypothesis were correct. AppendicesFinding percent of vitamin C each fruit contains with recommended daily vitamin C intake. Lime:1.3 mg ascorbic acid (250 mL)/60mg * vitamin C= 542 %Orange: 1.02 mg ascorbic acid (250 mL)/60mg * one nose candy= 425 %Mango:0.5 mg ascorbic acid (250 mL)/60mg * one hundred= 208 %Tomato:0.44 mg ascorbic acid (250 mL)/60mg *100= 183 %Apple:0.18 mg ascorbic acid (250 mL)/60mg *100= 75 %ReferencesGordon, D. J. (n.d.). HowStuff workings How Vitamin C Works. Retrieved June 11, 2009, from http://www.howstuffworks.com/vitamin-c.htmMedlinePlus Herbs and Supplements: Vitamin C (ascorbic acid) . (n.d.). Retrieved June 11, 2009, from http://www.nlm.nih.gov/medlineplus/druginfo/natural/patient-vitaminc.htmlVitamin C and Citrus Juices. (n.d.). Retrieved June 11, 2009, from http://www.ultimatecitrus.com/vitaminc.html If you want to pass a full essay, order it on our website: Ordercustompaper.com

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